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产品图片
货号/SKU
MRA-263
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-263?? Hybridoma 20G8 Anti-Aedes aegypti Salivary Glands(Cell Banks)|Aedes aegypti|Hybridoma 20G8 Anti-Aedes aegypti Salivary Glands|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|KD VernickAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Hybridoma 20G8 Anti-Aedes aegypti Salivary Glands, MRA-263, contributed by Kenneth D. Vernick."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The murine hybridoma cell line, 20G8, was generated by the fusion of SP2/0 mouse myeloma cells with splenocytes from BALB/c mice immunized with salivary gland membranes of Aedes aegypti (Ae. aegypti), strain Black-Eye Liverpool mosquitoes. The monoclonal antibody produced binds preferentially to the distal lateral lobes of female and male Ae. aegypti salivary glands; it also recognizes ovarian tissue. The 20G8 antibody does not cross-react with salivary glands of Anopheles gambiae but does with those of Culex pipiens mosquitoes.
Hybridoma 20G8 produces monoclonal antibody of the IgG2a subclass, which is specific for female and male Ae. aegypti salivary glands, and is reported to function in immunoblot and immunofluorescence assays.
Each vial contains approximately 0.5 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of 106 cells per mL. Sufficient cells are provided to initiate at least one new culture.
The murine hybridoma cell line, 20G8, was generated by the fusion of SP2/0 mouse myeloma cells with splenocytes from BALB/c mice immunized with salivary gland membranes of Aedes aegypti (Ae. aegypti), strain Black-Eye Liverpool mosquitoes. The monoclonal antibody produced binds preferentially to the distal lateral lobes of female and male Ae. aegypti salivary glands; it also recognizes ovarian tissue. The 20G8 antibody does not cross-react with salivary glands of Anopheles gambiae but does with those of Culex pipiens mosquitoes.
Hybridoma 20G8 produces monoclonal antibody of the IgG2a subclass, which is specific for female and male Ae. aegypti salivary glands, and is reported to function in immunoblot and immunofluorescence assays.
Each vial contains approximately 0.5 mL of hybridoma cells in cell culture medium supplemented with 10% dimethylsulfoxide (DMSO) at a concentration of 106 cells per mL. Sufficient cells are provided to initiate at least one new culture.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
鼠杂交瘤细胞系20G8由SP2 / 0小鼠骨髓瘤细胞的融合产生，其中BALB / C小鼠的脾细胞与 AEGESAEGYPTI（AE的唾液腺膜免疫）免疫的BALB / C小鼠.菌株黑色 - 利物浦蚊子.产生的单克隆抗体优先于雌性和雄性的远侧侧瓣粘合. Aegypti 唾液腺;它还识别卵巢组织. 20G8抗体不会与 anophelesGambiae的唾液腺体交叉反应，但与蚊子的 Culex pipiens 蚊子一起进行.
杂交瘤20G8产生IgG2A亚类的单克隆抗体，其特异性对雌性和雄性和雄性. AEGYPTI 唾液腺，并据报道，在免疫印迹和免疫荧光测定中起作用.
每个小瓶在细胞培养基中含有大约0.5ml杂交瘤细胞，其补充有10％二甲基磺砜（DMSO），其浓度为每mL 10 6℃的细胞.提供足够的细胞以引发至少一种新培养物.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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