纯化的甘露聚糖 (LM)(抗原制剂），NR-1_Vaccinia virus

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14850 is a preparation of the lipomannan (LM) derived from the cell wall of irradiated Mycobacterium tuberculosis, strain H37Rv.

Mycobacterium tuberculosis, strain H37Rv was grown to late-log phase in glycerol-alanine-salts, washed with PBS and inactivated by gamma irradiation. The cells were delipidated, suspended in buffer containing 4% Triton X-114 and broken by French Press. Insoluble material was removed from the lysate by repeated centrifugation. The Triton X-114 extract was collected, heated to allow biphasic partitioning and centrifuged. The detergent layer was collected and macromolecules, including LM, were recovered by ethanol precipitation. The ethanol insoluble material was suspended in PBS, and the proteins were digested and dialyzed out. The crude carbohydrate mixture was fractionated by size exclusion chromatography and the pure LM pooled. Buffer contaminants were removed by extensive dialysis. Contaminating LPS was avoided as all buffers and water used were endotoxin-free.

Each vial contains approximately 100 ?g of lyophilized purified LM from Mycobacterium tuberculosis, strain H37Rv. Lyophilized products may aggregate as a loose powder near the lid. Please take precautionary measures (such as tapping the bottom of the tube on the lab bench) to reduce escape of the reagent when opening the vial to reconstitute contents.