- 4 次围观
产品图片
产品别名
货号/SKU
EVM102
货号/规格
50ug
库存与交货期
1-2周
人民币价格
9640
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Christopher S. Francklyn, PhD, University of Vermont.
产品描述信息
Product Type: | Protein |
Name: | Histidyl-tRNA Synthetase Recombinant Protein w/6x His |
Accession ID: | AAC75567, P60906 |
Source: | E. coli |
Molecular Weight: | 47 kDa |
Amino Acid Sequence: | MGRSHHHHHHmakniqairgmndylpgetaiwqriegtlknvlgsygyseirlpiveqtplfkraigevtdvvekemytfedrngdsltlrpegtagcvragiehgllynqeqrlwyigpmfrherpqkgryrqfhqlgcevfglqgpdidaelimltarwwralgisehvtlelnsigslearanyrdalvafleqhkekldedckrrmytnplrvldsknpevqallndapalgdyldeesrehfaglckllesagiaytvnqrlvrgldyynrtvfewvtnslgsqgtvcaggrydglveqlggratpavgfamglerlvllvqavnpefkadpvvdiylvasgadtqsaamalaerlrdelpgvklmtnhgggnfkkqfaradkwgarvavvlgesevangtavvkdlrsgeqtavaqdsvaahlrtllg |
Fusion Tag(s): | N-terminal 6x His-tag |
Purity: | > 95% by HisTrap |
Buffer: | 50 mM HEPES pH 7.5; 100 mM KCl; 10 mM MgCl2, 5 mM ?-mercaptoethanol, 40% glycerol |
Concentration: | 100 uM |
Storage: | Store at -20C |
Shipped: | Dry ice |
产品安全信息
Francklyn, C. and Schimmel, P., Enzymatic aminoacylation of an eight-base-pair microhelix with histidine. PNAS, 87, 8655-8659, (1990). Franckyln, C., Harris, D., and Moras, D., Crystallization of Histidyl-tRNA Synthase from Escherichia coli, Journal of Molecular Biology, 241, 275-277, (1994). Yan, W. and Francklyn, C. Cytosine 73 is a discriminator nucleotide in vivo for histidyl-tRNA in Escherchia coli. Journal of Biological Chemistry, 269:13, 10022-10027 (1994). Bovee, M.L., Yan, W., Sproat, B.S., and Francklyn, C.S. tRNA discrimination at the binding step by a class II aminoacyl-tRNA synthetase. Biochemistry, 38: 41, 13725-13735 (1999). Arnez, J.G., Harris, D.C., Mitschler, A., Reese, B., Francklyn, C.S., and Moras, D. Crystal structure of histidyl-tRNA synthetase from Escherichia coli complexed with histidyl-adenylate. EMBO Journal, 14:17, 4143-4155 (1995). Yan, W., Augustine, J., and Francklyn, C. A tRNA identity switch mediated by the binding interaction between a tRNA anticodon and the accessory domain of a class II aminoacyl-tRNA synthetase. Biochemistry, 35:21, 6559-6568 (1996). Arnez, J.G., Augustine, J.G., Moras, D., and Francklyn, C. The first step of aminoacylation at the atomic level in histidyl-tRNA synthase. PNAS, 94, 7144-7149 (1997). Francklyn, C., Adams, J., and Augustine, J. Catalytic defects in mutants of class II histidyl-tRNA synthetase from Salmonella typhimurium previously linked to decreased control of histidine biosynthesis regulation. Journal of Molecular Biology, 280, 847-858 (1998). Francklyn, C., Musier-Forsyth, K., and Martinis, S.A. Aminoacyl-tRNA synthetases in biology and disease: new evidence for structural and functional diversity in an ancient family of enzymes RNA, 3, 945-960 (1997). Hawko, S.A., and Francklyn, C.S. Covariation of a specificity-determining structural motif in an aminoacyl-tRNA synthetase and a tRNA identity element. Biochemistry, 40:7, 1930-1936 (2001). Connoly, S.A., Rosen, A.E., Musier-Forsyth, K., and Francklyn, C.S. G-1:C73 recognition by an arginine cluster in the active site of Escherichia coli histidyl-tRNA synthetase. Biochemistry, 43: 4, 962-969 (2004). Guth, E., Connoly, S.H., Bovee, M., and Francklyn, C.S. A substrate-assisted concerted mechanism for aminoacylation by a class II aminoacyl-tRNA synthetase. Biochemistry, 44:10, 3785-3794 (2005) Guth, E. and Francklyn, C.S. Kinetic discrimination of tRNA identity by the conserved motif 2 loop of a class II aminoacyl-tRNA synthetase. Molecular Cell, 25, 531-542 (2007). Francklyn, C.S., First, E.A., Perona, J.J., and Hou, Y. Methods for kinetic and thermodynamic analysis of aminoacyl-tRNA synthetases. Methods, 44:2, 100-118 (2008). If you publish research with this product, please let us know so we can cite your paper.
主要内容
全长大肠杆菌组氨基-TRNA合成酶(HARS)已经使用Histrap亲和层析纯化,含有N-末端6x多氨酸标签.Histidyl-TRNA合成酶在其同源转移RNA的3'末端附着氨基酸ATP催化的两步反应。该反应的准确性对于蛋白质合成的整体保真度至关重要。 Hars中的缺陷是eMER综合征3b(USH3B)[MIM:614504]的原因。 USH3B是一种综合征,其特征在于幼儿期间的渐进视力和听力损失。有些患者有所谓的“查尔斯帽综合症”,涉及减少的视力和生动的视觉幻觉。 USH是一种基因上的异质条件,其特征在于视网膜炎与感官耳聋的视网膜炎。在发病和视觉和前庭功能的差异的年龄区分迎来亚瑟综合征1(USH1),映综合征2(USH2)和迎膜综合征3(USH3)。 USH3的特点是后期,进展性听力损失,可变性的心脏功能障碍,以及视网膜炎症状的发作,包括尼克罗缺乏,视野的收缩,以及中枢视力的丧失,通常是克里斯托弗的实验室弗里蒙大学博士,博士。调查员附属物计划的部分。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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