- 2 次围观
产品图片
产品别名
货号/SKU
EUV102
货号/规格
100ug
库存与交货期
咨询客服
人民币价格
21175
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Philip M. Carpenter, MD, University of Southern California.
产品描述信息
Product Type: | Protein |
Name: | Human Laminin 332 (Alternative names: laminin 5,laminin V, epiligrin, kalinin, ladsin, nicein). |
Accession ID: | Alpha 3: Q16787, NP_937762.1; Beta 3: Q13751, NP_000219.2; Gamma 2: Q13753, NM_005562 |
Source: | 184A1 human mammary epithelial cell conditioned media |
Molecular Weight: | 450 kDa, unprocessed |
Amino Acid Sequence: | Sequences available upon request. |
Fusion Tag(s): | None |
Purity: | Approximately 90%, Assessed by SDS PAGE. |
Buffer: | 150 mM NaCl, 10 mM Tris, pH 7.4, filter sterile |
Stability: | Stable when kept at -20C (or lower), at the concentration provided. IMPORTANT: Rapidly lose in ability to provide cell detachment when stored after being diluted. It is suggested to thaw once into aliquots prior to dilution and diluting the aliquots immediately as needed for experiments. Aliquots should be stored in sterile polycarbonate ependorff tubes. |
Concentration: | See product packaging. Concentration determined by OD280 of a standard curve of Bovine Seum Albumin (Concentration ≈ OD280 x 1.788) |
Comments: | Filter Sterile, both processed and unprocessed forms are present. |
Storage: | -20C or lower (Thawed protein retains activity for 1 month if stored at 4 C). |
Shipped: | Dry ice |
产品安全信息
Aumailley M, Bruckner-Tuderman L, Carter WG, et al. A simplified laminin nomenclature. Matrix Biol. 2005;24(5):326-332.Goldfinger LE, Hopkinson SB, deHart GW, Collawn S, Couchman JR, Jones JC. The alpha-3 laminin subunit, alpha-6-beta-4 and alpha-3-beta-1 integrin coordinately regulate wound healing in cultured epithelial cells and in the skin. J Cell Sci. 1999;112(Pt 16):2615-2629.Patarroyo M, Tryggvason K, Virtanen I. Laminin isoforms in tumor invasion, angiogenesis and metastasis. Semin Cancer Biol 2002;12(3):197-207Carpenter PM, Dao AV, Arain ZS, et al. Motility induction in breast carcinoma by mammary epithelial laminin 332 (laminin 5). Mol Cancer Res. 2009;7(4):462-75.Guess CM, Quaranta V. Defining the role of laminin-332 in carcinoma. Matrix Biol. 2009;28(8):445-55.Carpenter PM, Sivadas P, Hua SS, Xiao C, Gutierrez AB, Ngo T, Gershon PD. Migration of breast cancer cell lines in response to pulmonary laminin 332. Cancer Med. 2016 Nov 22. doi: 10.1002/cam4.957. View ArticleMuranen T, Iwanicki MP, Curry NL, Hwang J, DuBois CD, Coloff JL, Hitchcock DS, Clish CB, Brugge JS, Kalaany NY. Starved epithelial cells uptake extracellular matrix for survival. Nat Commun. 2017 Jan 10;8:13989. Cell Rep. 2017 Jan 10;18(2):334-343. View ArticleWang H, Jin H, Beauvais DM, Rapraeger AC. Cytoplasmic domain interactions of syndecan-1 and syndecan-4 with α6β4 integrin mediate human epidermal growth factor receptor (HER1 and HER2)-dependent motility and survival. J Biol Chem. 2014 Oct 31;289(44):30318-32. doi: 10.1074/jbc.M114.586438. PubMed PMID: 25202019; PubMed Central PMCID: PMC4215216. View ArticleCarpenter PM, Ziogas A, Markham EM, Cantillep AS, Yan R, Anton-Culver H. Laminin 332 expression and prognosis in breast Cancer. Hum Pathol. 2018 Aug 17. pii: S0046-8177(18)30307-1. View ArticleChen M, Choi S, Jung O, Wen T, Baum C, Thapa N, Lambert PF, Rapraeger AC, Anderson RA. The Specificity of EGF-Stimulated IQGAP1 Scaffold Towards the PI3K-Akt Pathway is Defined by the IQ3 motif. Sci Rep. 2019 Jun 24;9(1):9126. View ArticleFouillen A, Grenier D, Barbeau J, Baron C, Moffatt P, Nanci A. Selective bacterial degradation of the extracellular matrix attaching the gingiva to the tooth. Eur J Oral Sci. 2019 Aug;127(4):313-322. View ArticleNakhaei K, Ishijima M, Ikeda T, Ghassemi A, Saruta J, Ogawa T. Ultraviolet Light Treatment of Titanium Enhances Attachment, Adhesion, and Retention of Human Oral Epithelial Cells via Decarbonization. Materials (Basel). 2020 Dec 31;14(1):151.View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
通过亲和力和尺寸排阻色谱法从永生化的184A1乳腺上皮细胞的条件培养基中纯化该人层蛋白332蛋白。纳米蛋白332(以前是层粘连蛋白5)是由α3,β3和γ2链组成的α3,β3和γ2链蛋白。它在皮肤,乳腺和其他组织中表达,其中涉及形成血液染色体和伤口愈合。层内蛋白332涉及癌症进展。在乳腺癌中,层粘连蛋白332已被鉴定为微环境蛋白,其可以充当运动因子。从南加州大学菲利普M. Carpenter的实验室。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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