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产品图片
产品别名
货号/SKU
EUR302
货号/规格
100ug
库存与交货期
1-2周
人民币价格
10615
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Wei Hsu, PhD, University of Rochester.
产品描述信息
Product Type: | Antibody |
Antigen: | Wntless/Gpr177 |
Molecular Weight: | ~60 kDa |
Clonality: | Polyclonal |
Reactivity: | Human, mouse and rat (others not tested) |
Immunogen: | peptide |
Species Immunized: | Rabbit |
Epitope: | CDGPTEIYKLTRKEAQE |
Purification Method: | Affinity purified with peptide column |
Method Used to Determine Concentration: | OD 280 |
Buffer: | PBS |
Tested Applications: | WB: (1:1,000), IF: (1:1,000), IHC-parrafin (1:3,000) |
Concentration: | 2.53 mg/mL |
Storage: | -80C |
Shipped: | Dry ice |
产品安全信息
Fu J, Jiang M, Mirando AJ, Yu HM, Hsu W. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation." Proceedings of the National Academy of Sciences of the United States of America. 2009 Nov 3; 106(44):18598-603.Bänziger C, Soldini D, Schütt C, Zipperlen P, Hausmann G, Basler K. Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells. Cell. 2006 May 5;125(3):509-22.Bartscherer K, Pelte N, Ingelfinger D, Boutros M. Secretion of Wnt ligands requires Evi, a conserved transmembrane protein. Cell. 2006 May 5;125(3):523-33.Yu HM, Jin Y, Fu J, Hsu W. "Expression of Gpr177, a Wnt trafficking regulator, in mouse embryogenesis." Developmental dynamics : an official publication of the American Association of Anatomists. 2010 Jul; 239(7):2102-9.Fu J, Ivy Yu HM, Maruyama T, Mirando AJ, Hsu W. "Gpr177/mouse Wntless is essential for Wnt-mediated craniofacial and brain development." Developmental dynamics : an official publication of the American Association of Anatomists. 2011 Feb; 240(2):365-71. Epub 2011 Jan 11.Fu J, Hsu W. "Epidermal Wnt controls hair follicle induction by orchestrating dynamic signaling crosstalk between the epidermis and dermis." The Journal of investigative dermatology. 2013 Apr; 133(4):890-8.Maruyama T, Jiang M, Hsu W. "Gpr177, a novel locus for bone mineral density and osteoporosis, regulates osteogenesis and chondrogenesis in skeletal development." Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013 May; 28(5):1150-9.Maruyama EO, Yu HM, Jiang M, Fu J, Hsu W. "Gpr177 deficiency impairs mammary development and prohibits Wnt-induced tumorigenesis." PloS one. 2013 8(2):e56644.Zhu X, Zhao P, Liu Y, Zhang X, Fu J, Yu HM, Qiu M, Chen Y, Hsu W, Zhang Z. "Intra-epithelial requirement of canonical Wnt signaling for tooth morphogenesis." The Journal of biological chemistry. 2013 Apr 26; 288(17):12080-9.Zhu XJ, Liu Y, Dai ZM, Zhang X, Yang X, Li Y, Qiu M, Fu J, Hsu W, Chen Y, Zhang Z. "BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin." PLoS genetics. 2014 Oct; 10(10):e1004687.Pan CL, Baum PD, Gu M, Jorgensen EM, Clark SG, Garriga G. C. elegans AP-2 and retromer control Wnt signaling by regulating mig-14/Wntless. Dev Cell. 2008 Jan;14(1):132-9.Yang PT, Lorenowicz MJ, Silhankova M, Coudreuse DY, Betist MC, Korswagen HC. Wnt signaling requires retromer-dependent recycling of MIG-14/Wntless in Wnt-producing cells. Dev Cell. 2008 Jan;14(1):140-7.Belenkaya TY, Wu Y, Tang X, Zhou B, Cheng L, Sharma YV, Yan D, Selva EM, Lin X. The retromer complex influences Wnt secretion by recycling wntless from endosomes to the trans-Golgi network. Dev Cell. 2008 Jan;14(1):120-31.Port F, Kuster M, Herr P, Furger E, Bänziger C, Hausmann G, Basler K. Wingless secretion promotes and requires retromer-dependent cycling of Wntless. Nat Cell Biol. 2008 Feb;10(2):178-85.Franch-Marro X, Wendler F, Guidato S, Griffith J, Baena-Lopez A, Itasaki N, Maurice MM, Vincent JP. Wingless secretion requires endosome-to-Golgi retrieval of Wntless/Evi/Sprinter by the retromer complex. Nat Cell Biol. 2008 Feb;10(2):170-7.Stefater JA 3rd, Lewkowich I, Rao S, Mariggi G, Carpenter AC, Burr AR, Fan J, Ajima R, Molkentin JD, Williams BO, Wills-Karp M, Pollard JW, Yamaguchi T, Ferrara N, Gerhardt H, Lang RA. Regulation of angiogenesis by a non-canonical Wnt-Flt1 pathway in myeloid cells. Nature. 2011 May 29;474(7352):511-5.Landin Malt A, Hogan AK, Smith CD, Madani MS, Lu X. Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling. J Cell Biol. 2020 Oct 5;219(10):e201912071. View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
该兔子多克隆抗体是针对合成肽产生的,并且对于人,小鼠和大鼠G蛋白偶联受体177的序列Cdgpteykltrkeaqe(wntless / gpr177)的反应性是反应性的.highlights:与人,小鼠和大鼠Wntless / gpr177反应WED蛋白偶联受体177(GPR177)的Western印迹,免疫荧光和免疫组化应用程序是Wnt-Thermla(WLS; AKA EVI,SRT)的哺乳动物同源物是WNT分泌物中的WNT分泌[1-3]。 Wntless与脂质改性的Wnts结合,主要是在Golgi和贩运囊泡中的局部化以调节Wnt蛋白的细胞内分选[1]。已经证明,Wntless在小鼠中的遗传失活导致典型Wnt [4-10]的丧失以及非规范Wnt信号传导的类似缺陷[16]。然而,Wntless的丧失不会影响WNT表达,但在信号产生细胞中的分泌物[1,6]。 Wntless也与回力复合物相关联,以从等离子体膜中回收Wnt [11-15]。 Wntless的表达模式为生产和疾病中WNT的生成细胞和WNT来源提供了关键信息。从罗切斯特大学魏恒,博士的实验室。部分调查员的附件计划。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
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