- 252 次围观
产品图片
产品别名
货号/SKU
EKC004
货号/规格
1 vial
库存与交货期
1-2周
人民币价格
22600
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Lynda F. Bonewald, PhD, University of Missouri - Kansas City.
产品描述信息
Product Type: | Cell Line |
Name: | IDG-SW3 |
Cell Type: | Osteoblast/early osteocyte/late osteocyte |
Accession ID: | CVCL_0P23 |
Organism: | Mouse |
Source: | Long bone |
Morphology: | Adherent osteoblast-like cell |
Biosafety Level: | I |
Subculturing: | See: IDG-SW3 Cell Line Protocol |
Growth Conditions: |
Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; 1% penicillin-streptomycin; Recombinant Mouse Interferon-gamma (INF-γ) 50 U/ml. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Incubate at 33°C with 5% CO2 Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; 1% penicillin-streptomycin; 50µg/ml Ascorbic Acid and 4mM β-glycerophosphate. Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. Incubate at 37°C with 8% CO2. |
Cryopreservation: | 60% AlphaMEM, 30% FBS, 10% DMSO, at 1-2 x 10^6 cells/vial/1ml |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
产品安全信息
Woo SM, Rosser J, Dusevich V, Kalajzic I, Bonewald LF. Cell line IDG-SW3 replicates osteoblast-to-late-osteocyte differentiation in vitro and accelerates bone formation in vivo. J Bone Miner Res. 2011 Nov;26(11):2634-46.Ito N, Findlay DM, Anderson PH, Bonewald LF, Atkins GJ. Extracellular phosphate modulates the effect of 1?,25-dihydroxy vitamin D3 (1,25D) on osteocyte like cells J Steroid Biochem Mol Biol. 2013 Jul;136:183-6.St John HC, Bishop KA, Meyer MB, Benkusky NA, Leng N, Kendziorski C, Bonewald LF, Pike JW. The osteoblast to osteocyte transition: epigenetic changes and response to the vitamin d3 hormone. Mol Endocrinol. 2014 Jul;28(7):1150-65.Fujita K, Xing Q, Khosla S, Monroe DG. Mutual Enhancement of Differentiation of Osteoblasts and Osteocytes Occurs Through Direct Cell-Cell Contact. J Cell Biochem. 2014 Jul 15.Shao J, Zhou Y, Lin J, Nguyen TD, Huang R, Gu Y, Friis T, Crawford R, Xiao Y. Notch expressed by osteocytes plays a critical role in mineralisation. J Mol Med (Berl). 2018 Feb 17. View ArticleShao J, Zhou Y, Xiao Y. The regulatory roles of Notch in osteocyte differentiation via the crosstalk with canonical Wnt pathways during the transition of osteoblasts to osteocytes. Bone. 2018 Mar;108:165-178. View ArticleGiacomino CM, Wealleans JA, Kuhn N, Diogenes A. Comparative Biocompatibility and Osteogenic Potential of Two Bioceramic Sealers. J Endod. 2019 Jan;45(1):51-56. View ArticleBär L, Hase P, Föller M. PKC regulates the production of fibroblast growth factor 23 (FGF23). PLoS One. 2019 Mar 28;14(3):e0211309. View ArticleZhou Y, Lin J, Shao J, Zuo Q, Wang S, Wolff A, Nguyen DT, Rintoul L, Du Z, Gu Y, Peng YY, Ramshaw JAM, Long X, Xiao Y. Aberrant activation of Wnt signaling pathway altered osteocyte mineralization. Bone. 2019 Jun 28;127:324-333. View ArticleHingorani DV, Camargo MF, Quraishi MA, Adams SR, Advani SJ. Tumor Activated Cell Penetrating Peptides to Selectively Deliver Immune Modulatory Drugs. Pharmaceutics. 2021 Mar 10;13(3):365.View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
细胞系IDG-SW3将骨赘复制到体外后骨细胞分化,并增加体内骨形成.Highlights:从早期到后骨细胞的表达系列标记,包括:DMP1-GFP,E11 / GP38,SOST / SCLEROSTIN和FGF-23维持在33°CWITH的非分化状态下,IFN-g存在 - 允许大规模生产,而不会在在37℃下培养时作为原代基因表达的表型相同的基因表达,在没有IFN-G存在克隆细胞 - 均匀的情况下,并且在可以在2D和3D培养物中保持相同的分化阶段,长期活力(35-50天)骨细胞是体内最丰富的骨细胞,但也是最具挑战性的研究,因为它们嵌入了矿化矩阵中的矿化矩阵难以孤立。该细胞系应该是有用的研究成骨细胞转变的骨细胞转变,生物醛骨质细胞功能的机制和SOST / SCLEROSTIN和FGF-23的调节。从密苏里大学Lynda F. Bonewald,博士学位的Lynda F. Bonewald实验室。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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