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产品图片

产品英文名称

Murine Osteocyte-like Cell Line MLO-Y4

产品别名

Kerafast独特的生物试剂

货号/SKU

EKC002

货号/规格

1 vial

库存与交货期

Unavailable

人民币价格

16285

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的展示的人民币价格已包含商品本身金额、VAT增值税13%、国际运输运费、国内物流运费、运输保险、以及冷链包装材料（例如液氮罐、泡沫箱、金属桶、蓝冰、湿冰、干冰、蓄冷剂、液氮等）、装卸费、相关资料费、人力支出等一切费用。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

使用人负责A/B风险申请资质

国外采购

支持/部分需签MTA

厂牌

Kerafast, Inc.

品牌

Kerafast®

产品基础信息

From the laboratory of Lynda Bonewald, PhD, University of Missouri - Kansas City.

产品描述信息

Product Type:	Cell Line
Name:	MLO-Y4
Cell Type:	Osteocyte
Organism:	Mouse (female)
Accession ID:	CVCL_M098
Morphology:	Dendritic morphology
Biosafety Level:	I
Subculturing:	Maintain stock cultures in complete culture medium at ~60-75% confluence on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:5 dilution (~1:7maximum) using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81
Growth Conditions:	alpha-MEM, containing L-glutamine and deoxyribonucleosides, with penicillin/streptomycin at 100 U/ml and 2.5% FBS and 2.5% CS; both heat inactivated; Grown on dishes coated with [0.15 mg/ml] rat tail type 1 collagen (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81).
Cryopreservation:	60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81)
Comments:	When first starting these cells, centrifuge at approx. 1,000 rpm, for 5–10 min. Aspirate media, gently resuspend and plate the cell pellet in media containing 5% FBS and 5% CS. This higher amount of serum, 10% total, is useful to give the cells an extra boost. The next day, check the viability of the cells. If there are a lot of fl oating “dead” cells, change the medium. You can revert back to medium with 2.5% FBS + 2.5% CS at this point.
Storage:	Liquid nitrogen
Shipped:	Dry ice

产品安全信息

Kato Y, Windle J,Koop B, Qiao M, Bonewald L. Establishment of an osteocyte-like cell line, MLO-Y4 J Bone Min. Res. 12:2014-2023, 1997.Kato, Y, Boskey, A, Spevak, L, Dallas, M, Hori, M, Bonewald, LF, Establishment of an Osteoid Pre-Osteocyte like cell, MLO-A5, that spontaneously mineralizes in culture without the addition of beta-glycerol phosphate and ascorbic acid. J. Bone Min. Res. 16:1622-1633, 2001.Rosser J, Bonewald LF. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Methods Mol Biol. 2012;816:67-81.Sun Y, Kaur K, Kanayama K, Morinaga K, Park S, Hokugo A, Kozlowska A, McBride WH, Li J, Jewett A, Nishimura I. Plasticity of Myeloid Cells during Oral Barrier Wound Healing and the Development of Bisphosphonate-Related Osteonecrosis of the Jaw. J Biol Chem. 2016 Aug 11. pii: jbc.M116.735795. View ArticleWang T, Han C, Tian P, Li PF, Ma XL. Role of Teriparatide in Glucocorticoid-induced Osteoporosis through Regulating Cellular Reactive Oxygen Species. Orthop Surg. 2018 May 10. View ArticleKim HJ, Kim HJ, Choi Y, Bae MK, Hwang DS, Shin SH, Lee JY. Zoledronate Enhances Osteocyte-Mediated Osteoclast Differentiation by IL-6/RANKL Axis. Int J Mol Sci. 2019 Mar 22;20(6). pii: E1467. View ArticleYamamoto H, Saito M, Goto T, Ueshima K, Ishida M, Hayashi S, Ikoma K, Mazda O, Kubo T. Heme oxygenase-1 prevents glucocorticoid and hypoxia-induced apoptosis and necrosis of osteocyte-like cells. Med Mol Morphol. 2019 Jan 31. View ArticleParmentier L, Riffault M, Hoey DA. Utilizing Osteocyte Derived Factors to Enhance Cell Viability and Osteogenic Matrix Deposition within IPN Hydrogels. Materials (Basel). 2020;13(7):1690. Published 2020 Apr 4. View articleUeda S, Shimasaki M, Ichiseki T, Hirata H, Kawahara N, Ueda Y. Mitochondrial Transcription Factor A added to Osteocytes in a Stressed Environment has a Cytoprotective Effect. Int J Med Sci. 2020;17(9):1293-1299. Published 2020 May 23.View articleEichholz KF, Woods I, Riffault M, et al. Human bone marrow stem/stromal cell osteogenesis is regulated via mechanically activated osteocyte-derived extracellular vesicles [published online ahead of print, 2020 Jul 16]. Stem Cells Transl Med. 2020;10.1002/sctm.19-0405.View articleHirata H, Ueda S, Ichiseki T, Shimasaki M, Ueda Y, Kaneuji A, Kawahara N. Taurine Inhibits Glucocorticoid-Induced Bone Mitochondrial Injury, Preventing Osteonecrosis in Rabbits and Cultured Osteocytes. Int J Mol Sci. 2020 Sep 20;21(18):E6892.View articleWang Z, Weng Y, Ishihara Y, Odagaki N, Ei Hsu Hlaing E, Izawa T, Okamura H, Kamioka H. Loading history changes the morphology and compressive force-induced expression of receptor activator of nuclear factor kappa B ligand/osteoprotegerin in MLO-Y4 osteocytes. PeerJ. 2020 Nov 9;8:e10244.View articleIf you publish research with this product, please let us know so we can cite your paper.

主要内容

MLO-Y4细胞系可用于研究流体流动和基板在骨细胞特异性信号传导途径的活化上的影响。高灯：从转基因小鼠衍生，其中在骨科蛋白启动子高表达的控制下表达了永生化T-抗原的骨钙素高表达Connexin 43和抗原E11保留了树突状形态，类似于原发性骨细胞细胞中观察到的是身体中最丰富的骨细胞，也是最具挑战性的研究，因为它们嵌入了矿化基质中，使它们变得困难孤立。该细胞系MLO-Y4使得研究骨细胞功能更容易。通过Lynda Bonewald，密苏里大学博士博士的实验室， - 堪萨斯城。部分调查员的附件计划。

厂牌介绍

关于Kerafast Inc.

Kerafast 是一家位于波士顿的试剂公司，其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等，其中许多在其他地方无法获得。自 2011 年成立以来，来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂，无需通过传统的材料转让协议流程即可快速获取材料。

我们处理提供实验室的所有销售和运输物流，并从每次销售中返还丰厚的特许权使用费。因此，我们帮助提供实验室节省时间和资源，同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂，同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区，他们贡献和获取Reagent for the Greater Good，以加速他们自己的研究以及整体科学进步。

2018 年，Kerafast 与Absolute Antibody合并，后者是一家总部位于英国的公司，其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起，共同致力于改善科学界可用的研究工具的选择。