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Staphylococcus
aureus (S. aureus) subsp. aureus,
transposon mutant NE92 was derived from S. aureus
subsp. aureus, strain JE2. Mutagenesis occurred through the use of the mariner-based transposon bursa aurealis resulting in an
erythromycin-resistant deletion strain of JE2. S.
aureus subsp. aureus, transposon
mutant NE92 was created by disruption of qoxA, one
of four quinol oxidase genes in the qoxABCD operon that encode for the terminal
oxidase cytochrome aa3.
S.
aureus subsp. aureus,
strain JE2 is a plasmid-cured
derivative of strain LAC that was isolated in 2002 from a skin and soft tissue
infection of an inmate in the Los Angeles County Jail in California, USA.
Strain JE2 is a methicillin-resistant S. aureus (MRSA) strain and is a USA300
isolate. USA300
isolates have the same MLST profile (ST 8), SCCmec (subtype IV), agr group
(I) and spa motif (MBQBLO) and
typically carry the Panton-Valentine leukocidin (PVL) toxin genes and the
arginine catabolic mobile element (ACME). USA300 is the most common cause of
community-associated MRSA infection and an increasing cause of
hospital-acquired infections.
The complete genome of the parental strain, JE2, is available
(GenBank: CP020619).
Each vial contains approximately 0.5 mL of
bacterial culture in Tryptic Soy broth containing 5 ?g/mL erythromycin supplemented
with 10% glycerol.
The Nebraska Transposon Mutant Library (NTML) was constructed in the laboratories of Dr. Ken Bayles and Dr. Paul Fey at the University of Nebraska Medical Center. Additional information is available at the NTML website.
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
