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Methods Mol Biol. 2026;3038:147-170. doi: 10.1007/978-1-0716-5292-3_10.
ABSTRACT
Through miniaturisation of enzymatic reactions, microfluorescence analyses enable the non-invasive assessment of embryo metabolism. Nutrient concentrations in embryo culture medium are quantified by ultramicrofluorescence (UMF) techniques, which utilise specialised constriction pipettes and a fluorescent microscope coupled with a photomultiplier tube and photometer, to quantitate nutrient levels in nanolitre or picolitre volumes of spent media. Here, the non-invasive method for analysis of embryo metabolism utilising spent culture media and UMF is described.
PMID:42681383 | DOI:10.1007/978-1-0716-5292-3_10
来源出处
Non-Invasive Assessment of Embryo Physiology
https://pubmed.ncbi.nlm.nih.gov/42681383/?utm_source=Other&utm_medium=rss&utm_c…