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Reprod Domest Anim. 2026 Jan;61(1):e70160. doi: 10.1111/rda.70160.
ABSTRACT
The main objective of this study was to assess the effects of donor age on the development of in vitro-derived sheep embryos. Ovaries were obtained after slaughter from cycling Polish Longwool ewes aged 1.5-3 years (Group Y-'young'; n = 14) or 8-9 years (Group O-'old'; n = 16). Cumulus-oocyte complexes were collected and subjected to in vitro maturation (IVM), followed by in vitro fertilisation (IVF) with fresh capacitated ram semen. The resultant embryos were then cultured and monitored with a time-lapse (TL) imaging system for up to 8 days (Group Y, n = 64 and Group O, n = 48). The timing of key developmental stages relative to the moment when the oocytes and sperm were combined and including cleavage divisions as well as morula and blastocyst formation was recorded. Both the cleavage (68.75%) and blastocyst formation (26.6%) rates were significantly higher in younger ewes compared with their older counterparts (50.0% and 10.4%, respectively), with Group Y zygotes showing fewer (p
PMID:41482989 | DOI:10.1111/rda.70160