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Endocrinology. 2025 Aug 4:bqaf126. doi: 10.1210/endocr/bqaf126. Online ahead of print.
ABSTRACT
Here we describe organoid cultures derived from pig foliate taste papillae in which the cellular heterogeneity of the lingual epithelium is preserved. Pig taste organoids were maintained long term (18 passages) and continued to express taste stem cell markers (LGR4, LGR6 and SOX2) and taste receptor cell (TRC) markers (CK20, ENTPD2, GNAT3 and OTOP1). We show insulin is necessary for optimum proliferation and differentiation of taste organoids. Some TRCs in the organoids contained insulin, and the insulin critical transcription factors MAFA and PAX4. However, we did not see any evidence of the critical glucose responsive PDX-1 expression either in the native tissue or in the organoids. We optimized differentiation conditions for TRC expression and separately for increased insulin protein content (6.5-fold, p
PMID:40755411 | DOI:10.1210/endocr/bqaf126