Derivation of Lung Organoids from Human-Induced Pluripotent Stem Cells for Respiratory Infection Studies

Methods Mol Biol. 2025 Jul 31. doi: 10.1007/7651_2025_660. Online ahead of print.

ABSTRACT

The development of human-induced pluripotent stem cell (hiPSC)-derived lung organoids has revolutionized the study of lung development, disease modeling, and infection systems. These three-dimensional structures recapitulate key features of human lung physiology, including epithelial and mesenchymal interactions, and provide a biologically relevant platform for studying viral infections, epithelial-specific immune responses, and therapeutic interventions. Here, we outline the stepwise derivation of lung organoids from hiPSCs, describe their characterization, and discuss their application in infection modeling.

PMID:40736634 | DOI:10.1007/7651_2025_660