Peng SW, Guo XY, Shang GG, Li J, Xu XY, You ML, Li P, Chen GQ. An Assessment of the Risks of Carcinogenicity Associated with Polyhydroxyalkanoates through an Analysis of DNA Aneuploid and Telomerase Activity. BIOMATERIALS 32(10): 2546-2555, 2011

Abstract Polyhydroxyalkanoates (PHA) are aliphatic polyesters synthesized by many bacteria. Because of their flexible mechanical strengths, superior elastic property, biodegradability and biocompatibility, PHA have been developed for applications as medical implants, drug delivery matrices, and devices to support cell growth. Lots of studies showed that PHA matrices improved cell proliferation and tissue regeneration. However, the possibility of whether rapid cell proliferation on PHA matrices will induce tumor formation is unclear. Here we confirmed that proliferating rat osteoblasts grown on films of various PHA including PHB, PHBV, P3HB4HB, PHBHHx and PHBVHHx did not lead to cancer induction at least for p8th. Cell proliferation was evaluated by the incorporation of 5-bromodeoxyuridine......