Ye J, Li JZ, Liu Y, Li XH, Yang TS, Ma XD, Li Q, Yao ZM, Li P. Cideb, an ER and lipid droplet-associated protein, mediates VLDL lipidation and maturation by interacting with apolipoprotein B. CELL METABOLISM 9: 177-190, 2009

Abstract Secretion of triacylglycerol-enriched very-low-density lipoproteins (VLDLs) from the liver is vital for maintaining plasma lipid homeostasis. However, the process of VLDL assembly and lipidation is not well characterized. Here, we observed that liver of Cideb null mice had higher levels of triacylglycerols accompanied by low level of VLDL secretion. Furthermore, VLDL particles secreted from hepatocytes of Cideb null mice have low levels of triacylglycerols but normal levels of apoB. We also observed that Cideb is localized to endoplasmic reticulum and lipid droplets. Importantly, we have identified apoB as a Cideb-interacting protein. By infecting adenoviruses expressing various Cideb truncations into hepatocytes of Cideb null mice, we found that Cideb requires both its apoB-bind......