Zhang M, Lin S,Song X, Liu J, Fu Y, Fu X, Chang Z, and Chen PR*, A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance, NATURE CHEMICAL BIOLOGY, accepted.

root 提交于 周日, 10/31/2021 - 01:47
Abstract Acid chaperones are essential factors in preserving the protein homeostasis for enteric pathogens to survive in the extremely acidic mammalian stomach (pH 1-3). The client proteins of these chaperones remain largely unknown, primarily because of the exceeding difficulty of determining protein-protein interactions under low-pH conditions. We developed a genetically encoded, highly efficient protein photocrosslinking probe, which enabled us to profile the in vivo substrates of a major acid-protection chaperone, HdeA, in Escherichia coli periplasm. Among the identified HdeA client proteins, the periplasmic chaperones DegP and SurA were initially found to be protected by HdeA at a low pH, but they subsequently facilitated the HdeA-mediated acid recovery of other client proteins. This......

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