Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X. p31comet blocks Mad2 activation through structural mimicry. CELL131(4):744-55, 2007

root 提交于 周日, 10/31/2021 - 01:47
Abstract The status of spindle checkpoint signaling depends on the balance of two opposing dynamic processes that regulate the highly unusual two-state behavior of Mad2. In mitosis, a Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding, thereby facilitating checkpoint activation. p31(comet) inactivates the checkpoint through binding to Mad1- or Cdc20-bound Mad2, thereby preventing Mad2 activation and promoting the dissociation of the Mad2-Cdc20 complex. Here, we report the crystal structure of the Mad2-p31(comet) complex. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of......

相关内容

发布日期 01/10/2022 - 19:31
发布日期 10/31/2021 - 01:16
发布日期 11/09/2024 - 19:46
发布日期 11/17/2024 - 19:48
发布日期 07/23/2023 - 21:46
发布日期 03/11/2025 - 20:51
发布日期 06/17/2022 - 10:21
发布日期 10/14/2023 - 23:10
发布日期 06/05/2024 - 17:45