Chen PR*, Bae T, Williams W.A., Duguid E.M., Rice P.A., Schneewind O, and He C, An oxidation sensing mechanism is used by a global regulator MgrA in Staphylococcus aureus, NATURE CHEMICAL BIOLOGY, 2, 591-595. 2006.

Abstract Staphylococcus aureus is a human pathogen responsible for most wound and hospital-acquired infections. The protein MgrA is both an important virulence determinant during infection and a regulator of antibiotic resistance in S. aureus. The crystal structure of the MgrA homodimer, solved at 2.86 A, indicates the presence of a unique cysteine residue located at the interface of the protein dimer. We discovered that this cysteine residue can be oxidized by various reactive oxygen species, such as hydrogen peroxide and organic hydroperoxide. Cysteine oxidation leads to dissociation of MgrA from DNA and initiation of signaling pathways that turn on antibiotic resistance in S. aureus. The oxidation-sensing mechanism is typically used by bacteria to counter challenges of reactive oxygen ......